1) which tool of recombinant dna technology is incorrectly paired with its use:
a) restriction enzyme: production of RFLPs (restriction fragment length polymorphism)
b) DNA ligase: enzskin analyzer amwayymes that cut DNA, creating sticky ends
c) DNA polymerase: used in PCR to amplify sections of DNA
d) Reverse transcriptase:…
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1) which tool of recombinant dna technology is incorrectly paired with its use:
a) restriction enzyme: production of RFLPs (restriction fragment length polymorphism)
b) DNA ligase: enzymes that cut DNA, creating sticky ends
c) DNA polymerase: used in PCR to amplify sections of DNA
d) Reverse transcriptase: production of cDNA from mRNA
e) electrophoresis: dna sequencing

2) a paleontologist recovered a bit of tissue from the preserved skin of an extinct dodo. The researcher would like to compare DNA from the sample to that of living birds. Which of the following would be the most useful in increasing the amount of dodo DNA available for testing?
a) RFLP (restriction fragment length polymorphism) analyzer
b) PCR
c) electroporation
d) Gel electrophoresis
e) southern hybridization

MATCH:

restriction endonucleases and Restriction fragments

which one has sticky ends?
which one cut DNA at restriction sites?

**10 points to the person giving lots of explanation*

Answer:
Maikang 1-b dna ligas link together DNA strands that have double-strand breaks NOT cut DNA

2-PCR- it amplifies dna
The polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA thus generated is itself used as template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. With PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece. PCR can be extensively modified to perform a wide array of genetic manipulations.

3- Restriction endonucleases Cuts DNA at restriction sites- is an enzyme
Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave at the end of a polynucleotide chain. Restriction endonucleases (Restriction Enzymes) cleave DNA at specific sites, and are divided into three categories, Type I, Type II, and Type III, according to their mechanism of action. These enzymes are often used in genetic engineering to make recombinant DNA for introduction into bacterial, plant, or animal cells.

4- restriction fragments- (which are already cut by restriction endonucleases) has sticky ends.skin analyzer

Maikang 1)a 2)d 3)c 4)dskin analyzer

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